PI3 Antibody

ProSci
Product Code: PSI-19-664
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-664-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 3
Western blot analysis of extracts of various cell lines, using PI3 antibody (19-664) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 3
Immunohistochemistry of paraffin-embedded human colon using PI3 antibody (19-664) at dilution of 1:100 (40x lens).
3 / 3
Immunohistochemistry of paraffin-embedded mouse lung using PI3 antibody (19-664) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using PI3 antibody (19-664) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human colon using PI3 antibody (19-664) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using PI3 antibody (19-664) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
PI3, ESI, MGC13613, SKALP, WAP3, WFDC14, cementoin
Application Note:
WB: 1:500 - 1:2000

IHC: 1:100 - 1:200
Background:
This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria, and fungal pathogens. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 23-117 of human PI3 (NP_002629.1).
NCBI Gene ID #:
5266
NCBI Official Name:
peptidase inhibitor 3, skin-derived
NCBI Official Symbol:
PI3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 12kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Immunology, Innate Immunity
Swissprot #:
P19957
User NOte:
Optimal dilutions for each application to be determined by the researcher.