HSD17B10 Antibody

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ProSci
Product Code: PSI-19-669
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-669-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using HSD17B10 antibody (19-669) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Western blot analysis of extracts of various cell lines, using HSD17B10 antibody (19-669) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded human tonsil using HSD17B10 antibody (19-669) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver cancer using HSD17B10 antibody (19-669) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon using HSD17B10 antibody (19-669) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U-2 OS cells using HSD17B10 Polyclonal Antibody (19-669) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using HSD17B10 antibody (19-669) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Western blot analysis of extracts of various cell lines, using HSD17B10 antibody (19-669) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human tonsil using HSD17B10 antibody (19-669) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using HSD17B10 antibody (19-669) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using HSD17B10 antibody (19-669) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-2 OS cells using HSD17B10 Polyclonal Antibody (19-669) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
HADH2, ABAD, ERAB, MHBD, HSD17B10, 17b-HSD10, hydroxyacyl-Coenzyme A dehydrogenase, type II, type 10 17b-HSD, AB-binding alcohol dehydrogenase, type 10 17beta-hydroxysteroid dehydrogenase, HADH2, MRPP2, SCHAD, XH98G2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene encodes 3-hydroxyacyl-CoA dehydrogenase type II, a member of the short-chain dehydrogenase/reductase superfamily. The gene product is a mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids and steroids, and is a subunit of mitochondrial ribonuclease P, which is involved in tRNA maturation. The protein has been implicated in the development of Alzheimer disease, and mutations in the gene are the cause of 17beta-hydroxysteroid dehydrogenase type 10 (HSD10) deficiency. Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human HSD17B10 (NP_004484.1).
NCBI Gene ID #:
3028
NCBI Official Name:
hydroxysteroid (17-beta) dehydrogenase 10
NCBI Official Symbol:
HSD17B10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 26kDa
Purification:
Affinity purification
Research Area:
Neuroscience
Swissprot #:
Q99714
User NOte:
Optimal dilutions for each application to be determined by the researcher.