FHL1 Antibody

ProSci
Product Code: PSI-19-678
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-678-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 4
Western blot analysis of extracts of various cell lines, using FHL1 antibody (19-678) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 4
Immunohistochemistry of paraffin-embedded human thyroid cancer using FHL1 Antibody (19-678) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded mouse kidney using FHL1 Antibody (19-678) at dilution of 1:100 (40x lens).
4 / 4
Immunofluorescence analysis of HeLa cells using FHL1 antibody (19-678). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using FHL1 antibody (19-678) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human thyroid cancer using FHL1 Antibody (19-678) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using FHL1 Antibody (19-678) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using FHL1 antibody (19-678). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
FHL1, FHL1B, KYO-T, MGC111107, SLIM1, bA535K18.1, KYOT, SLIM, FHL-1, FHL1A, FLH1A, XMPMA, SLIM-1, SLIMMER
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes. Mutations in this gene have been found in patients with Emery-Dreifuss muscular dystrophy. Multiple alternately spliced transcript variants which encode different protein isoforms have been described.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 27-296 of human FHL1 (NP_001153171.1).
NCBI Gene ID #:
2273
NCBI Official Name:
four and a half LIM domains 1
NCBI Official Symbol:
FHL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 40kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q13642
User NOte:
Optimal dilutions for each application to be determined by the researcher.