HIF1AN Antibody

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ProSci
Product Code: PSI-19-681
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-681-50uL50uL£440.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using HIF1AN antibody (19-681) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded rat brain using HIF1AN antibody (19-681) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human gastric cancer using HIF1AN antibody (19-681) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using HIF1AN antibody (19-681) at dilution of 1:100 (40x lens).
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Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug HIF1AN antibody (19-681). Western blot was performed from the immunoprecipitate using HIF1AN antibody (19-681) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using HIF1AN antibody (19-681) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat brain using HIF1AN antibody (19-681) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using HIF1AN antibody (19-681) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using HIF1AN antibody (19-681) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug HIF1AN antibody (19-681). Western blot was performed from the immunoprecipitate using HIF1AN antibody (19-681) at a dilition of 1:1000.

Documents

Further Information

Additional Names:
HIF1AN, DKFZp762F1811, FIH1, FLJ20615, FLJ22027
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

IP: 1:50 - 1:200
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-349 of human HIF1AN (NP_060372.2).
NCBI Gene ID #:
55662
NCBI Official Name:
hypoxia inducible factor 1, alpha subunit inhibitor
NCBI Official Symbol:
HIF1AN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 40kDa
Purification:
Affinity purification
Research Area:
Cancer
Swissprot #:
Q9NWT6
User NOte:
Optimal dilutions for each application to be determined by the researcher.