PRPF3 Antibody

ProSci
Product Code: PSI-19-691
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-691-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 5
Western blot analysis of extracts of various cell lines, using PRPF3 antibody (19-691) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 5
Immunohistochemistry of paraffin-embedded rat brain using PRPF3 Antibody (19-691) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded human stomach using PRPF3 Antibody (19-691) at dilution of 1:100 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded mouse brain using PRPF3 Antibody (19-691) at dilution of 1:100 (40x lens).
5 / 5
Immunofluorescence analysis of U2OS cells using PRPF3 antibody (19-691). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using PRPF3 antibody (19-691) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using PRPF3 Antibody (19-691) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using PRPF3 Antibody (19-691) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using PRPF3 Antibody (19-691) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using PRPF3 antibody (19-691). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
PRPF3, PRP3, RP18, HPRP3, Prp3p, HPRP3P, SNRNP90
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

IP: 1:50 - 1:100
Background:
The removal of introns from nuclear pre-mRNAs occurs on complexes called spliceosomes, which are made up of 4 small nuclear ribonucleoprotein (snRNP) particles and an undefined number of transiently associated splicing factors. This gene product is one of several proteins that associate with U4 and U6 snRNPs. Mutations in this gene are associated with retinitis pigmentosa-18.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PRPF3 (NP_004689.1).
NCBI Gene ID #:
9129
NCBI Official Name:
pre-mRNA processing factor 3
NCBI Official Symbol:
PRPF3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 100kDa
Purification:
Affinity purification
Research Area:
Neuroscience
Swissprot #:
O43395
User NOte:
Optimal dilutions for each application to be determined by the researcher.