PSRC1 Antibody

ProSci
Product Code: PSI-19-693
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-693-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PSRC1 antibody (19-693) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human esophagus using PSRC1 antibody (19-693) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse stomach using PSRC1 antibody (19-693) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using PSRC1 antibody (19-693) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human esophagus using PSRC1 antibody (19-693) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using PSRC1 antibody (19-693) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
DDA3, FP3214, proline/serine-rich coiled-coil protein 1, differential display and activated by p53, p53-regulated DDA3, proline/serine-rich coiled-coil 1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene encodes a proline-rich protein that is a target for regulation by the tumor suppressor protein p53. The encoded protein plays an important role in mitosis by recruiting and regulating microtubule depolymerases that destabalize microtubules. Alternatively spliced transcript variants encoding different isoforms have been described.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human PSRC1 (NP_001005290.1).
NCBI Gene ID #:
84722
NCBI Official Name:
proline and serine rich coiled-coil 1
NCBI Official Symbol:
PSRC1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 39kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle
Swissprot #:
Q6PGN9
User NOte:
Optimal dilutions for each application to be determined by the researcher.