TXNRD2 Antibody

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ProSci
Product Code: PSI-19-698
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-698-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using TXNRD2 antibody (19-698) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human liver damage using TXNRD2 antibody (19-698) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver cancer using TXNRD2 antibody (19-698) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using TXNRD2 antibody (19-698) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver damage using TXNRD2 antibody (19-698) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using TXNRD2 antibody (19-698) at dilution of 1:100 (40x lens).

Documents

Further Information

Additional Names:
GCCD5, SELZ, TR, TR-BETA, TR3, TRXR2, thioredoxin reductase 2, mitochondrial, selenoprotein Z, thioredoxin reductase 3, thioredoxin reductase TR3, thioredoxin reductase beta
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes a mitochondrial form important for scavenging reactive oxygen species in mitochondria. It functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternatively spliced transcript variants encoding different isoforms, including a few localized in the cytosol and some lacking the C-terminal Sec residue, have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 100-310 of human TXNRD2 (NP_006431.2).
NCBI Gene ID #:
10587
NCBI Official Name:
thioredoxin reductase 2
NCBI Official Symbol:
TXNRD2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 56kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
Q9NNW7
User NOte:
Optimal dilutions for each application to be determined by the researcher.