TRIM22 Antibody

ProSci
Product Code: PSI-19-706
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-706-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of A-549 cells, using TRIM22 antibody (19-706).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunofluorescence analysis of U2OS cells using TRIM22 antibody (19-706) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of A-549 cells, using TRIM22 antibody (19-706).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of U2OS cells using TRIM22 antibody (19-706) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
TRIM22, GPSTAF50, RNF94, STAF50
Application Note:
WB: 1:500 - 1:1000

IF: 1:50 - 1:100
Background:
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the cytoplasm and its expression is induced by interferon. The protein down-regulates transcription from the HIV-1 LTR promoter region, suggesting that function of this protein may be to mediate interferon's antiviral effects. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 215-411 of human TRIM22 (NP_006065.2).
NCBI Gene ID #:
10346
NCBI Official Name:
tripartite motif containing 22
NCBI Official Symbol:
TRIM22
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 57kDa
Purification:
Affinity purification
Research Area:
Chemokines & Cytokines, Immunology, Innate Immunity, Stem Cell
Swissprot #:
Q8IYM9
User NOte:
Optimal dilutions for each application to be determined by the researcher.