PRDM14 Antibody

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ProSci
Product Code: PSI-19-719
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-719-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of rat testis, using PRDM14 antibody (19-719) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
2 / 4
Immunofluorescence analysis of HeLa cells using PRDM14 antibody (19-719) at dilution of 1:100. Blue: DAPI for nuclear staining.
3 / 4
Immunofluorescence analysis of NIH/3T3 cells using PRDM14 antibody (19-719) at dilution of 1:100. Blue: DAPI for nuclear staining.
4 / 4
Chromatin immunoprecipitation analysis of RUNX1 gene from 293 cell line, using PRDM14 antibody (19-719) and rabbit IgG. P1, P2, P3 and P4 were four probes located on RUNX1 gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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Western blot analysis of extracts of rat testis, using PRDM14 antibody (19-719) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using PRDM14 antibody (19-719) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using PRDM14 antibody (19-719) at dilution of 1:100. Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of RUNX1 gene from 293 cell line, using PRDM14 antibody (19-719) and rabbit IgG. P1, P2, P3 and P4 were four probes located on RUNX1 gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Documents

Further Information

Additional Names:
PRDM14, PFM11
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

IP: 1:20 - 1:100

ChIP: 1:20 - 1:100
Background:
This gene encodes a member of the PRDI-BF1 and RIZ homology domain containing (PRDM) family of transcriptional regulators. The encoded protein may possess histone methyltransferase activity and plays a critical role in cell pluripotency by suppressing the expression of differentiation marker genes. Expression of this gene may play a role in breast cancer.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-571 of human PRDM14 (AAH52311.1).
NCBI Gene ID #:
63978
NCBI Official Name:
PR domain containing 14
NCBI Official Symbol:
PRDM14
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 64kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9GZV8
User NOte:
Optimal dilutions for each application to be determined by the researcher.