RNF2 Antibody

ProSci
Product Code: PSI-19-735
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-735-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using RNF2 antibody (19-735) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat lung using RNF2 antibody (19-735) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human gastric cancer using RNF2 antibody (19-735) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using RNF2 antibody (19-735) at dilution of 1:100 (40x lens).
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Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug RNF2 antibody (19-735). Western blot was performed from the immunoprecipitate using RNF2 antibody (19-735) at a dilition of 1:1000.
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Chromatin immunoprecipitation analysis of extracts of HeLa cells, using RNF2 antibody (19-735) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Western blot analysis of extracts of various cell lines, using RNF2 antibody (19-735) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat lung using RNF2 antibody (19-735) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using RNF2 antibody (19-735) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using RNF2 antibody (19-735) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug RNF2 antibody (19-735). Western blot was performed from the immunoprecipitate using RNF2 antibody (19-735) at a dilition of 1:1000.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using RNF2 antibody (19-735) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Further Information

Additional Names:
RNF2, BAP1, DING, BAP-1, HIPI3, RING2, RING1B
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

IP: 1:50 - 1:200

ChIP: 1:50 - 1:200
Background:
Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-336 of human RNF2 (NP_009143.1).
NCBI Gene ID #:
6045
NCBI Official Name:
ring finger protein 2
NCBI Official Symbol:
RNF2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 38kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q99496
User NOte:
Optimal dilutions for each application to be determined by the researcher.