WNK3 Antibody

ProSci
Product Code: PSI-19-741
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-741-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using WNK3 antibody (19-741).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded human liver cancer using WNK3 antibody (19-741) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded rat kidney using WNK3 antibody (19-741) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using WNK3 antibody (19-741).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human liver cancer using WNK3 antibody (19-741) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using WNK3 antibody (19-741) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
RGD1563131, serine/threonine-protein kinase WNK3
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a protein belonging to the 'with no lysine' family of serine-threonine protein kinases. These family members lack the catalytic lysine in subdomain II, and instead have a conserved lysine in subdomain I. This family member functions as a positive regulator of the transcellular Ca2+ transport pathway, and it plays a role in the increase of cell survival in a caspase-3-dependent pathway. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant protein of rat WNK3
NCBI Gene ID #:
317420
NCBI Official Name:
WNK lysine deficient protein kinase 3
NCBI Official Symbol:
WNK3
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 40kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
User NOte:
Optimal dilutions for each application to be determined by the researcher.