FUBP1 Antibody

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ProSci
Product Code: PSI-19-747
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-747-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using FUBP1 antibody (19-747) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human liver damage using FUBP1 antibody (19-747) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human lung cancer using FUBP1 antibody (19-747) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using FUBP1 antibody (19-747) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of C6 cells using FUBP1 antibody (19-747) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug FUBP1 antibody (19-747). Western blot was performed from the immunoprecipitate using FUBP1 antibody (19-747) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using FUBP1 antibody (19-747) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver damage using FUBP1 antibody (19-747) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using FUBP1 antibody (19-747) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using FUBP1 antibody (19-747) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using FUBP1 antibody (19-747) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug FUBP1 antibody (19-747). Western blot was performed from the immunoprecipitate using FUBP1 antibody (19-747) at a dilition of 1:1000.

Documents

Further Information

Additional Names:
FUBP1, FBP, FUBP
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100

IP: 1:50 - 1:100
Background:
The protein encoded by this gene is a single stranded DNA-binding protein that binds to multiple DNA elements, including the far upstream element (FUSE) located upstream of c-myc. Binding to FUSE occurs on the non-coding strand, and is important to the regulation of c-myc in undifferentiated cells. This protein contains three domains, an amphipathic helix N-terminal domain, a DNA-binding central domain, and a C-terminal transactivation domain that contains three tyrosine-rich motifs. The N-terminal domain is thought to repress the activity of the C-terminal domain. This protein is also thought to bind RNA, and contains 3'-5' helicase activity with in vitro activity on both DNA-DNA and RNA-RNA duplexes. Aberrant expression of this gene has been found in malignant tissues, and this gene is important to neural system and lung development. Binding of this protein to viral RNA is thought to play a role in several viral diseases, including hepatitis C and hand, foot and mouth disease. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 302-644 of human FUBP1 (NP_003893.2).
NCBI Gene ID #:
8880
NCBI Official Name:
far upstream element (FUSE) binding protein 1
NCBI Official Symbol:
FUBP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 79kDa
Purification:
Affinity purification
Research Area:
Transcription
Swissprot #:
Q96AE4
User NOte:
Optimal dilutions for each application to be determined by the researcher.