HPX Antibody

ProSci
Product Code: PSI-19-752
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-752-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using HPX antibody (19-752) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat testis using HPX antibody (19-752) at dilution of 1:100 (20x lens).
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Immunohistochemistry of paraffin-embedded mouse testis using HPX antibody (19-752) at dilution of 1:100 (20x lens).
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Immunofluorescence analysis of U2OS cells using HPX antibody (19-752). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using HPX antibody (19-752) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat testis using HPX antibody (19-752) at dilution of 1:100 (20x lens).
Immunohistochemistry of paraffin-embedded mouse testis using HPX antibody (19-752) at dilution of 1:100 (20x lens).
Immunofluorescence analysis of U2OS cells using HPX antibody (19-752). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Hemopexin, Beta-1B-glycoprotein, HPX
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:10 - 1:100
Background:
This gene encodes a plasma glycoprotein that binds heme with high affinity. The encoded protein is an acute phase protein that transports heme from the plasma to the liver and may be involved in protecting cells from oxidative stress.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 24-245 of human HPX (NP_000604.1).
NCBI Gene ID #:
3263
NCBI Official Name:
Hemopexin
NCBI Official Symbol:
HPX
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 68kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Signal Transduction
Swissprot #:
P02790
User NOte:
Optimal dilutions for each application to be determined by the researcher.