FLT4 Antibody

ProSci
Product Code: PSI-19-753
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-753-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of mouse liver, using FLT4 antibody (19-753) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human colon using FLT4 antibody (19-753) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung using FLT4 antibody (19-753) at dilution of 1:200 (40x lens).
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Immunofluorescence analysis of U2OS cells using FLT4 antibody (19-753) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of mouse liver, using FLT4 antibody (19-753) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human colon using FLT4 antibody (19-753) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using FLT4 antibody (19-753) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using FLT4 antibody (19-753) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
FLT4, FLT41, Fms-related tyrosine kinase 4, Fms-like tyrosine kinase 4, LMPH1A, PCL, Soluble VEGFR3 variant 1, Vegf-r3, VEGFR3, Soluble VEGFR3 variant 2, Soluble VEGFR3 variant 3, FLT-4, Quek2, VEGF Receptor 3, VEGFR-3
Application Note:
WB: 1:500 - 1:1000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. Mutations in this gene cause hereditary lymphedema type IA.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1174-1363 of human FLT4 (NP_891555.2).
NCBI Gene ID #:
2324
NCBI Official Name:
fms-related tyrosine kinase 4
NCBI Official Symbol:
FLT4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 153kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Growth Factors, Signal Transduction
Swissprot #:
P35916
User NOte:
Optimal dilutions for each application to be determined by the researcher.