CREM Antibody

ProSci
Product Code: PSI-19-762
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-762-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CREM antibody (19-762) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 180s.
2 / 3
Immunohistochemistry of paraffin-embedded human mammary cancer using CREM antibody (19-762) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human placenta using CREM antibody (19-762) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using CREM antibody (19-762) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 180s.
Immunohistochemistry of paraffin-embedded human mammary cancer using CREM antibody (19-762) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using CREM antibody (19-762) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
CREM, ICER, CREM-2, hCREM-2, RP11-324I22.3
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a bZIP transcription factor that binds to the cAMP responsive element found in many viral and cellular promoters. It is an important component of cAMP-mediated signal transduction during the spermatogenetic cycle, as well as other complex processes. Alternative promoter and translation initiation site usage allows this gene to exert spatial and temporal specificity to cAMP responsiveness. Multiple alternatively spliced transcript variants encoding several different isoforms have been found for this gene, with some of them functioning as activators and some as repressors of transcription.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CREM (NP_853549.1).
NCBI Gene ID #:
1390
NCBI Official Name:
cAMP responsive element modulator
NCBI Official Symbol:
CREM
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 39kDa
Purification:
Affinity purification
Research Area:
Neuroscience, Signal Transduction, Transcription
Swissprot #:
Q03060
User NOte:
Optimal dilutions for each application to be determined by the researcher.