CTNNA1 Antibody

ProSci
Product Code: PSI-19-768
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-768-50uL50uL£440.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CTNNA1 antibody (19-768) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded rat kidney using CTNNA1 antibody (19-768) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human vermiform appendix using CTNNA1 antibody (19-768) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using CTNNA1 antibody (19-768) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse testis using CTNNA1 antibody (19-768) at dilution of 1:100 (40x lens).
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Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug CTNNA1 antibody (19-768). Western blot was performed from the immunoprecipitate using CTNNA1 antibody (19-768) at a dilition of 1:500.

Western blot analysis of extracts of various cell lines, using CTNNA1 antibody (19-768) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat kidney using CTNNA1 antibody (19-768) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human vermiform appendix using CTNNA1 antibody (19-768) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using CTNNA1 antibody (19-768) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using CTNNA1 antibody (19-768) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug CTNNA1 antibody (19-768). Western blot was performed from the immunoprecipitate using CTNNA1 antibody (19-768) at a dilition of 1:500.

Further Information

Additional Names:
Catenin alpha-1, Alpha E-catenin, Cadherin-associated protein, Renal carcinoma antigen NY-REN-13, CTNNA1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IP: 1:50 - 1:100
Background:
This gene encodes a member of the catenin family of proteins that play an important role in cell adhesion process by connecting cadherins located on the plasma membrane to the actin filaments inside the cell. The encoded mechanosensing protein contains three vinculin homology domains and undergoes conformational changes in response to cytoskeletal tension, resulting in the reconfiguration of cadherin-actin filament connections. Certain mutations in this gene cause butterfly-shaped pigment dystrophy.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 637-906 of human CTNNA1 (NP_001894.2).
NCBI Gene ID #:
1495
NCBI Official Name:
catenin (cadherin-associated protein), alpha 1, 102kDa
NCBI Official Symbol:
CTNNA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 115kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Signal Transduction, Stem Cell
Swissprot #:
P35221
User NOte:
Optimal dilutions for each application to be determined by the researcher.