ECE1 Antibody

ProSci
Product Code: PSI-19-770
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-770-50uL50uL£440.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of mouse liver, using ECE1 antibody (19-770) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded rat spleen using ECE1 antibody (19-770) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human mammary cancer using ECE1 antibody (19-770) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using ECE1 antibody (19-770) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of L929 cells using ECE1 Polyclonal Antibody (19-770) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of mouse liver, using ECE1 antibody (19-770) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat spleen using ECE1 antibody (19-770) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human mammary cancer using ECE1 antibody (19-770) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using ECE1 antibody (19-770) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of L929 cells using ECE1 Polyclonal Antibody (19-770) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Endothelin-converting enzyme 1, ECE-1, ECE1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
The protein encoded by this gene is involved in proteolytic processing of endothelin precursors to biologically active peptides. Mutations in this gene are associated with Hirschsprung disease, cardiac defects and autonomic dysfunction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 670-770 of human ECE1 (NP_001388.1).
NCBI Gene ID #:
1889
NCBI Official Name:
Endothelin-converting enzyme 1
NCBI Official Symbol:
ECE1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 130kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Growth Factors, Signal Transduction
Swissprot #:
P42892
User NOte:
Optimal dilutions for each application to be determined by the researcher.