FLI1 Antibody

Contact us
ProSci
Product Code: PSI-19-775
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-775-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 4
Western blot analysis of extracts of Jurkat cells, using FLI1 antibody (19-775) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 4
Immunohistochemistry of paraffin-embedded human lung cancer using FLI1 antibody (19-775) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded human uterine cancer using FLI1 antibody (19-775) at dilution of 1:100 (40x lens).
4 / 4
Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 1ug FLI1 antibody (19-775). Western blot was performed from the immunoprecipitate using FLI1 antibody (19-775) at a dilition of 1:1000.
❮❯

Western blot analysis of extracts of Jurkat cells, using FLI1 antibody (19-775) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human lung cancer using FLI1 antibody (19-775) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterine cancer using FLI1 antibody (19-775) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 1ug FLI1 antibody (19-775). Western blot was performed from the immunoprecipitate using FLI1 antibody (19-775) at a dilition of 1:1000.

Documents

Further Information

Additional Names:
FLI1 Antibody: EWSR2, SIC-1, BDPLT21
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IP: 1:50 - 1:200
Background:
This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human FLI1 (NP_002008.2).
NCBI Gene ID #:
2313
NCBI Official Name:
Fli-1 proto-oncogene, ETS transcription factor
NCBI Official Symbol:
FLI1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 53kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Transcription
Swissprot #:
Q01543
User NOte:
Optimal dilutions for each application to be determined by the researcher.