EPO Antibody

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ProSci
Product Code: PSI-19-780
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-780-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using EPO antibody (19-780) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human esophagus using EPO antibody (19-780) (40x lens).
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Immunohistochemistry of paraffin-embedded rat liver using EPO antibody (19-780) (40x lens).
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Immunohistochemistry of paraffin-embedded human liver damage using EPO antibody (19-780) (40x lens).
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Western blot analysis of extracts of various cell lines, using EPO antibody (19-780) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human esophagus using EPO antibody (19-780) (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using EPO antibody (19-780) (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using EPO antibody (19-780) (40x lens).

Documents

Further Information

Additional Names:
Erythropoietin, Epoetin, EPO
Application Note:
WB: 1:500 - 1:2000

IHC: 1:100 - 1:200
Background:
This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide of human EPO
NCBI Gene ID #:
2056
NCBI Official Name:
erythropoietin
NCBI Official Symbol:
EPO
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 30kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Chemokines & Cytokines, Growth Factors, Immunology, Signal Transduction, Stem Cell
Swissprot #:
P01588
User NOte:
Optimal dilutions for each application to be determined by the researcher.