EFNB2 Antibody

ProSci
Product Code: PSI-19-783
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-783-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of mouse lung, using EFNB2 antibody (19-783) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded rat brain using EFNB2 antibody (19-783) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using EFNB2 antibody (19-783) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using EFNB2 antibody (19-783). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of mouse lung, using EFNB2 antibody (19-783) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat brain using EFNB2 antibody (19-783) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using EFNB2 antibody (19-783) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using EFNB2 antibody (19-783). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
EFNB2, MGC126228, MGC126227, MGC126226, ligand of eph-related kinase 5, LERK5, Htk-L, HTKL, HTK ligand, EPLG5, ephrin-B2, ephrin B2, eph-related receptor tyrosine kinase ligand 5
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:10 - 1:100
Background:
This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 28-229 of human EFNB2 (NP_004084.1).
NCBI Gene ID #:
1948
NCBI Official Name:
ephrin-B2
NCBI Official Symbol:
EFNB2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 50kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Growth Factors, Neuroscience
Swissprot #:
P52799
User NOte:
Optimal dilutions for each application to be determined by the researcher.