DOK1 Antibody

ProSci
Product Code: PSI-19-794
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-794-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 5
Western blot analysis of extracts of various cell lines, using DOK1 antibody (19-794) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 5
Immunohistochemistry of paraffin-embedded rat brain using DOK1 Antibody (19-794) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded human gastric cancer using DOK1 Antibody (19-794) at dilution of 1:100 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded mouse heart using DOK1 Antibody (19-794) at dilution of 1:100 (40x lens).
5 / 5
Immunofluorescence analysis of HeLa cells using DOK1 antibody (19-794). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using DOK1 antibody (19-794) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using DOK1 Antibody (19-794) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using DOK1 Antibody (19-794) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using DOK1 Antibody (19-794) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using DOK1 antibody (19-794). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
DOK1, MGC117395, MGC138860, P62DOK
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
The protein encoded by this gene is part of a signal transduction pathway downstream of receptor tyrosine kinases. The encoded protein is a scaffold protein that helps form a platform for the assembly of multiprotein signaling complexes. Several transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human DOK1 (NP_001372.1).
NCBI Gene ID #:
1796
NCBI Official Name:
docking protein 1, 62kDa (downstream of tyrosine kinase 1)
NCBI Official Symbol:
DOK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 52kDa
Purification:
Affinity purification
Research Area:
Cancer, Immunology, Obesity, Signal Transduction
Swissprot #:
Q99704
User NOte:
Optimal dilutions for each application to be determined by the researcher.