HPRT1 Antibody, KO Validated

ProSci
Product Code: PSI-19-798
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-798-50uL50uL£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using HPRT1 antibody (19-798) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
2 / 3
Western blot analysis of extracts from normal (control) and HPRT1 knockout (KO) 293T cells, using HPRT1 antibody (19-798) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
3 / 3
Immunofluorescence analysis of U2OS cells using HPRT1 antibody (19-798). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using HPRT1 antibody (19-798) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Western blot analysis of extracts from normal (control) and HPRT1 knockout (KO) 293T cells, using HPRT1 antibody (19-798) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunofluorescence analysis of U2OS cells using HPRT1 antibody (19-798). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
HPRT1, HGPRT, HPRT
Application Note:
WB: 1:500 - 1:2000

IF: 1:10 - 1:100
Background:
The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-218 of human HPRT1 (NP_000185.1).
NCBI Gene ID #:
3251
NCBI Official Name:
hypoxanthine phosphoribosyltransferase 1
NCBI Official Symbol:
HPRT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 27kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
P00492
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.