CD98 Antibody

ProSci
Product Code: PSI-19-804
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-804-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CD98 antibody (19-804) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 25s.
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Immunohistochemistry of paraffin-embedded human breast cancer using CD98 antibody (19-804) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat liver using CD98 antibody (19-804) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using CD98 antibody (19-804) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of HeLa cells using CD98 Polyclonal Antibody (19-804) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH-3T3 cells using CD98 Polyclonal Antibody (19-804) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using CD98 antibody (19-804) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 25s.
Immunohistochemistry of paraffin-embedded human breast cancer using CD98 antibody (19-804) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using CD98 antibody (19-804) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using CD98 antibody (19-804) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using CD98 Polyclonal Antibody (19-804) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using CD98 Polyclonal Antibody (19-804) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
4F2 cell-surface antigen heavy chain, 4F2hc, 4F2 heavy chain antigen, Lymphocyte activation antigen 4F2 large subunit, Solute carrier family 3 member 2, CD98, SLC3A2, MDU1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene is a member of the solute carrier family and encodes a cell surface, transmembrane protein. The protein exists as the heavy chain of a heterodimer, covalently bound through di-sulfide bonds to one of several possible light chains. The encoded transporter plays a role in regulation of intracellular calcium levels and transports L-type amino acids. Alternatively spliced transcript variants, encoding different isoforms, have been characterized.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 360-529 of human CD98 (NP_001013269.1).
NCBI Gene ID #:
6520
NCBI Official Name:
solute carrier family 3 (amino acid transporter heavy chain), member 2
NCBI Official Symbol:
SLC3A2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 100kDa
Purification:
Affinity purification
Research Area:
Cancer, Immunology, Neuroscience, Signal Transduction, Stem Cell
Swissprot #:
P08195
User NOte:
Optimal dilutions for each application to be determined by the researcher.