GPD1 Antibody

ProSci
Product Code: PSI-19-810
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-810-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using GPD1 antibody (19-810) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded rat heart using GPD1 Antibody (19-810) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat heart using GPD1 Antibody (19-810) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast adipocytes using GPD1 Antibody (19-810) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using GPD1 antibody (19-810) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat heart using GPD1 Antibody (19-810) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using GPD1 Antibody (19-810) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast adipocytes using GPD1 Antibody (19-810) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
GPD1, FLJ26652, GPD-C, HTGTI, GPDH-C
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a member of the NAD-dependent glycerol-3-phosphate dehydrogenase family. The encoded protein plays a critical role in carbohydrate and lipid metabolism by catalyzing the reversible conversion of dihydroxyacetone phosphate (DHAP) and reduced nicotine adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+. The encoded cytosolic protein and mitochondrial glycerol-3-phosphate dehydrogenase also form a glycerol phosphate shuttle that facilitates the transfer of reducing equivalents from the cytosol to mitochondria. Mutations in this gene are a cause of transient infantile hypertriglyceridemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-349 of human GPD1 (NP_005267.2).
NCBI Gene ID #:
2819
NCBI Official Name:
glycerol-3-phosphate dehydrogenase 1 (soluble)
NCBI Official Symbol:
GPD1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 38kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
P21695
User NOte:
Optimal dilutions for each application to be determined by the researcher.