PDLIM5 Antibody

ProSci
Product Code: PSI-19-814
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-814-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PDLIM5 antibody (19-814) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunofluorescence analysis of U2OS cells using PDLIM5 antibody (19-814). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using PDLIM5 antibody (19-814) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of U2OS cells using PDLIM5 antibody (19-814). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
PDLIM5, ENH, ENH1, L9, LIM
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
This gene encodes a member of a family of proteins that possess a 100-amino acid PDZ domain at the N terminus and one to three LIM domains at the C-terminus. This family member functions as a scaffold protein that tethers protein kinases to the Z-disk in striated muscles. It is thought to function in cardiomyocyte expansion and in restraining postsynaptic growth of excitatory synapses. Alternative splicing of this gene results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 317-596 of human PDLIM5 (NP_006448.4).
NCBI Gene ID #:
10611
NCBI Official Name:
PDZ and LIM domain 5
NCBI Official Symbol:
PDLIM5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 68kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience, Signal Transduction
Swissprot #:
Q96HC4
User NOte:
Optimal dilutions for each application to be determined by the researcher.