PRPF31 Antibody

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ProSci
Product Code: PSI-19-821
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-821-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PRPF31 antibody (19-821) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat heart using PRPF31 antibody (19-821) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human gastric cancer using PRPF31 antibody (19-821) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using PRPF31 antibody (19-821) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using PRPF31 antibody (19-821) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat heart using PRPF31 antibody (19-821) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using PRPF31 antibody (19-821) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using PRPF31 antibody (19-821) at dilution of 1:100 (40x lens).

Documents

Further Information

Additional Names:
pre-mRNA processing factor 31 homolog, PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae), Protein 61K, pre-mRNA processing factor 31 homolog (yeast), DKFZp566J153, NY-BR-99, PRP31, RP11, PRP31 pre-mRNA processing factor 31 homolog (yeast), PRPF31
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
This gene encodes a component of the spliceosome complex and is one of several retinitis pigmentosa-causing genes. When the gene product is added to the spliceosome complex, activation occurs.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PRPF31 (NP_056444.3).
NCBI Gene ID #:
26121
NCBI Official Name:
pre-mRNA processing factor 31
NCBI Official Symbol:
PRPF31
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 56kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q8WWY3
User NOte:
Optimal dilutions for each application to be determined by the researcher.