ACTR2 Antibody

ProSci
Product Code: PSI-19-823
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-823-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ACTR2 antibody (19-823) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat brain using ACTR2 antibody (19-823) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human uterine cancer using ACTR2 antibody (19-823) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using ACTR2 antibody (19-823) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using ACTR2 antibody (19-823). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using ACTR2 antibody (19-823) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using ACTR2 antibody (19-823) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterine cancer using ACTR2 antibody (19-823) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using ACTR2 antibody (19-823) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using ACTR2 antibody (19-823). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
ACTR2, ARP2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Two transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human ACTR2 (NP_005713.1).
NCBI Gene ID #:
10097
NCBI Official Name:
ARP2 actin-related protein 2 homolog (yeast)
NCBI Official Symbol:
ACTR2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 45kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Immunology, Signal Transduction
Swissprot #:
P61160
User NOte:
Optimal dilutions for each application to be determined by the researcher.