EZH2 Antibody

ProSci
Product Code: PSI-19-829
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-829-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using EZH2 antibody (19-829) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded rat lung using EZH2 Antibody (19-829) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using EZH2 Antibody (19-829) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using EZH2 antibody (19-829) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat lung using EZH2 Antibody (19-829) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using EZH2 Antibody (19-829) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
EZH2, WVS, ENX1, EZH1, KMT6, WVS2, ENX-1, EZH2b, KMT6A
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human EZH2 (NP_001190176.1).
NCBI Gene ID #:
2146
NCBI Official Name:
enhancer of zeste homolog 2 (Drosophila)
NCBI Official Symbol:
EZH2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 105kDa
Purification:
Affinity purification
Research Area:
Cancer
Swissprot #:
Q15910
User NOte:
Optimal dilutions for each application to be determined by the researcher.