SMARCB1 Antibody

ProSci
Product Code: PSI-19-845
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-845-50uL50uL£440.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using SMARCB1 antibody (19-845) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
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Immunohistochemistry of paraffin-embedded rat Intestine using SMARCB1 antibody (19-845) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse osteosarcoma using SMARCB1 antibody (19-845) at dilution of 1:200 (40x lens).

Western blot analysis of extracts of various cell lines, using SMARCB1 antibody (19-845) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat Intestine using SMARCB1 antibody (19-845) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse osteosarcoma using SMARCB1 antibody (19-845) at dilution of 1:200 (40x lens).

Further Information

Additional Names:
SMARCB1, RDT, INI1, SNF5, Snr1, BAF47, MRD15, RTPS1, Sfh1p, hSNFS, SNF5L1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor, and mutations in it have been associated with malignant rhabdoid tumors. Alternatively spliced transcript variants have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 77-376 of human SMARCB1 (NP_001007469.1).
NCBI Gene ID #:
6598
NCBI Official Name:
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1
NCBI Official Symbol:
SMARCB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 44kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle
Swissprot #:
Q12824
User NOte:
Optimal dilutions for each application to be determined by the researcher.