ATP5B Antibody

ProSci
Product Code: PSI-19-846
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-846-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ATP5B Antibody (19-846) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3s.
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Immunohistochemistry of paraffin-embedded rat heart using ATP5B antibody (19-846) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human lung cancer using ATP5B antibody (19-846) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human gastric cancer using ATP5B antibody (19-846) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using ATP5B antibody (19-846) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of HeLa cells using ATP5B antibody (19-846) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH/3T3 cells using ATP5B antibody (19-846) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of PC-12 cells using ATP5B antibody (19-846) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using ATP5B Antibody (19-846) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3s.
Immunohistochemistry of paraffin-embedded rat heart using ATP5B antibody (19-846) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using ATP5B antibody (19-846) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using ATP5B antibody (19-846) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using ATP5B antibody (19-846) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using ATP5B antibody (19-846) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using ATP5B antibody (19-846) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using ATP5B antibody (19-846) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
ATP5B, ATPMB, ATPSB, MGC5231, HEL-S-271
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 230-529 of human ATP5B (NP_001677.2).
NCBI Gene ID #:
506
NCBI Official Name:
ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
NCBI Official Symbol:
ATP5B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 57kDa
Purification:
Affinity purification
Research Area:
Neuroscience, Signal Transduction
Swissprot #:
P06576
User NOte:
Optimal dilutions for each application to be determined by the researcher.