TOPBP1 Antibody

ProSci
Product Code: PSI-19-856
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-856-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of 293T cells, using TOPBP1 antibody (19-856) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 50s.
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Immunohistochemistry of paraffin-embedded mouse heart using TOPBP1 antibody (19-856) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of A549 cells using TOPBP1 antibody (19-856). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of 293T cells, using TOPBP1 antibody (19-856) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 50s.
Immunohistochemistry of paraffin-embedded mouse heart using TOPBP1 antibody (19-856) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using TOPBP1 antibody (19-856). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Dpb11, TOP2BP1, DNA topoisomerase 2-binding protein 1, DNA topoisomerase II-beta-binding protein 1, topoisomerase (DNA) II binding protein 1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:100 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a binding protein which interacts with the C-terminal region of topoisomerase II beta. This interaction suggests a supportive role for this protein in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1253-1522 of human TOPBP1 (NP_008958.2).
NCBI Gene ID #:
11073
NCBI Official Name:
DNA topoisomerase II binding protein 1
NCBI Official Symbol:
TOPBP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 171kDa
Purification:
Affinity purification
Research Area:
Cancer
Swissprot #:
Q92547
User NOte:
Optimal dilutions for each application to be determined by the researcher.