CXXC1 Antibody

ProSci
Product Code: PSI-19-875
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-875-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CXXC1 antibody (19-875) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded mouse lung using CXXC1 antibody (19-875) at dilution of 1:200 (40x lens).
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Immunofluorescence analysis of A549 cells using CXXC1 antibody (19-875). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using CXXC1 antibody (19-875) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse lung using CXXC1 antibody (19-875) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of A549 cells using CXXC1 antibody (19-875). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
CXXC1, 2410002I16Rik, 5830420C16Rik, CFP1, CGBP, HsT2645, PCCX1, PHF18, SPP1, hCGBP, ZCGPC1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a protein that functions as a transcriptional activator that binds specifically to non-methylated CpG motifs through its CXXC domain. The protein is a component of the SETD1 complex, regulates gene expression and is essential for vertebrate development.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human CXXC1 (NP_001095124.1).
NCBI Gene ID #:
30827
NCBI Official Name:
CXXC finger protein 1
NCBI Official Symbol:
CXXC1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 75kDa
Purification:
Affinity purification
Research Area:
Transcription
Swissprot #:
Q9P0U4
User NOte:
Optimal dilutions for each application to be determined by the researcher.