DDX20 Antibody

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ProSci
Product Code: PSI-19-877
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-19-877-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using DDX20 antibody (19-877) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat kidney using DDX20 Antibody (19-877) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human amygdalitis using DDX20 Antibody (19-877) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon using DDX20 Antibody (19-877) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of HeLa cells using DDX20 antibody (19-877). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using DDX20 antibody (19-877) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using DDX20 Antibody (19-877) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human amygdalitis using DDX20 Antibody (19-877) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using DDX20 Antibody (19-877) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using DDX20 antibody (19-877). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
Probable ATP-dependent RNA helicase DDX20, Component of gems 3, DEAD box protein 20, DEAD box protein DP 103, Gemin-3, DDX20, DP103, GEMIN3
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:10 - 1:100
Background:
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which has an ATPase activity and is a component of the survival of motor neurons (SMN) complex. This protein interacts directly with SMN, the spinal muscular atrophy gene product, and may play a catalytic role in the function of the SMN complex on RNPs.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 25-280 of human DDX20 (NP_009135.4).
NCBI Gene ID #:
11218
NCBI Official Name:
Probable ATP-dependent RNA helicase DDX20
NCBI Official Symbol:
DDX20
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 110kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Neuroscience, Signal Transduction
Swissprot #:
Q9UHI6
User NOte:
Optimal dilutions for each application to be determined by the researcher.