GOT1 Antibody

ProSci
Product Code: PSI-19-881
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-881-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using GOT1 antibody (19-881) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded rat heart using GOT1 antibody (19-881) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human lung cancer using GOT1 antibody (19-881) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon using GOT1 antibody (19-881) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using GOT1 antibody (19-881). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using GOT1 antibody (19-881) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat heart using GOT1 antibody (19-881) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using GOT1 antibody (19-881) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using GOT1 antibody (19-881) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using GOT1 antibody (19-881). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
GOT1, GIG18, cCAT, cAspAT, ASTQTL1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:10 - 1:100
Background:
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 244-413 of human GOT1 (NP_002070.1).
NCBI Gene ID #:
2805
NCBI Official Name:
glutamic-oxaloacetic transaminase 1, soluble
NCBI Official Symbol:
GOT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 46kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
P17174
User NOte:
Optimal dilutions for each application to be determined by the researcher.