KDM4B Antibody

ProSci
Product Code: PSI-19-883
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-883-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using KDM4B antibody (19-883) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.
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Immunofluorescence analysis of U2OS cells using KDM4B antibody (19-883). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using KDM4B antibody (19-883) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.
Immunofluorescence analysis of U2OS cells using KDM4B antibody (19-883). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
JMJD2B, TDRD14B, lysine-specific demethylase 4B, jmjC domain-containing histone demethylation protein 3B, jumonji domain containing 2B, jumonji domain-containing protein 2B, lysine (K)-specific demethylase 4B, tudor domain containing 14B
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human KDM4B (NP_055830.1).
NCBI Gene ID #:
23030
NCBI Official Name:
lysine demethylase 4B
NCBI Official Symbol:
KDM4B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 110kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Immunology, Signal Transduction
Swissprot #:
O94953
User NOte:
Optimal dilutions for each application to be determined by the researcher.