PRKCQ Antibody

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ProSci
Product Code: PSI-19-889
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-889-50uL50uL£433.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PRKCQ Antibody (19-889) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human esophageal cancer using PRKCQ antibody (19-889) at dilution of 1:200 (40x lens).
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Western blot analysis of extracts of various cell lines, using PRKCQ Antibody (19-889) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human esophageal cancer using PRKCQ antibody (19-889) at dilution of 1:200 (40x lens).

Documents

Further Information

Additional Names:
Protein kinase C theta type, nPKC-theta, PRKCQ, PRKCT
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human PRKCQ (NP_006248.1).
NCBI Gene ID #:
5588
NCBI Official Name:
Protein kinase C theta type
NCBI Official Symbol:
PRKCQ
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 82kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Immunology, Signal Transduction
Swissprot #:
Q04759
User NOte:
Optimal dilutions for each application to be determined by the researcher.