TGM3 Antibody

ProSci
Product Code: PSI-19-901
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-901-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using TGM3 antibody (19-901) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 50s.
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Immunofluorescence analysis of U2OS cells using TGM3 antibody (19-901). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using TGM3 antibody (19-901) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 50s.
Immunofluorescence analysis of U2OS cells using TGM3 antibody (19-901). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
TGM3, transglutaminase-3, transglutaminase E, transglutaminase 3 (E polypeptide, protein-glutamine-gamma-glutamyltransferase), TGE, TGase-3, TGase E, TG(E), protein-glutamine gamma-glutamyltransferase E, MGC126250, MGC126249
Application Note:
WB: 1:500 - 1:2000

IF: 1:10 - 1:100
Background:
Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene consists of two polypeptide chains activated from a single precursor protein by proteolysis. The encoded protein is involved the later stages of cell envelope formation in the epidermis and hair follicle.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human TGM3 (NP_003236.3).
NCBI Gene ID #:
7053
NCBI Official Name:
transglutaminase 3
NCBI Official Symbol:
TGM3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 72kDa
Purification:
Affinity purification
Research Area:
Cancer, Neuroscience, Signal Transduction
Swissprot #:
Q08188
User NOte:
Optimal dilutions for each application to be determined by the researcher.