WNT2 Antibody

ProSci
Product Code: PSI-19-905
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-905-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of SKOV3 cells, using WNT2 Antibody (19-905) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded rat brain using WNT2 antibody (19-905) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver damage using WNT2 antibody (19-905) at dilution of 1:200 (40x lens).

Western blot analysis of extracts of SKOV3 cells, using WNT2 Antibody (19-905) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat brain using WNT2 antibody (19-905) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using WNT2 antibody (19-905) at dilution of 1:200 (40x lens).

Further Information

Additional Names:
INT1L1, IRP, protein Wnt-2, Int-1-related protein, epididymis secretory sperm binding protein, int-1-like protein 1, secreted growth factor, wingless-type MMTV integration site family member 2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene is a member of the WNT gene family. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Alternatively spliced transcript variants have been identified for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 26-360 of human WNT2 (NP_003382.1).
NCBI Gene ID #:
7472
NCBI Official Name:
Wnt family member 2
NCBI Official Symbol:
WNT2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 56kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Signal Transduction, Stem Cell
Swissprot #:
P09544
User NOte:
Optimal dilutions for each application to be determined by the researcher.