SF3B2 Antibody

Contact us
ProSci
Product Code: PSI-19-911
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-911-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 5
Western blot analysis of extracts of various cell lines, using SF3B2 antibody (19-911) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
2 / 5
Immunohistochemistry of paraffin-embedded rat heart using SF3B2 antibody (19-911) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded human lung cancer using SF3B2 antibody (19-911) at dilution of 1:100 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded mouse Intestine using SF3B2 antibody (19-911) at dilution of 1:100 (40x lens).
5 / 5
Immunofluorescence analysis of A-549 cells using SF3B2 antibody (19-911).
❮❯

Western blot analysis of extracts of various cell lines, using SF3B2 antibody (19-911) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat heart using SF3B2 antibody (19-911) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using SF3B2 antibody (19-911) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse Intestine using SF3B2 antibody (19-911) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A-549 cells using SF3B2 antibody (19-911).

Documents

Further Information

Additional Names:
Splicing factor 3B subunit 2, Pre-mRNA-splicing factor SF3b 145 kDa subunit, SF3b145, SF3b150, Spliceosome-associated protein 145, SAP 145, SF3B2, SAP145
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 616-895 of human SF3B2 (NP_006833.2).
NCBI Gene ID #:
10992
NCBI Official Name:
Splicing factor 3B subunit 2
NCBI Official Symbol:
SF3B2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 140kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q13435
User NOte:
Optimal dilutions for each application to be determined by the researcher.