RPS10 Antibody

ProSci
Product Code: PSI-19-914
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-914-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using RPS10 antibody (19-914) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded rat brain using RPS10 antibody (19-914) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using RPS10 antibody (19-914) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of MCF7 cells using RPS10 antibody (19-914). Blue: DAPI for nuclear staining.
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Immunoprecipitation analysis of 200ug extracts of MCF-7 cells using 1ug RPS10 antibody (19-914). Western blot was performed from the immunoprecipitate using RPS10 antibody (19-914) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using RPS10 antibody (19-914) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat brain using RPS10 antibody (19-914) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using RPS10 antibody (19-914) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using RPS10 antibody (19-914). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of MCF-7 cells using 1ug RPS10 antibody (19-914). Western blot was performed from the immunoprecipitate using RPS10 antibody (19-914) at a dilition of 1:1000.

Further Information

Additional Names:
40S ribosomal protein S10, RPS10
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:20 - 1:50

IP: 1:20 - 1:50
Background:
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S10E family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternate splicing results in multiple transcript variants that encode the same protein. Naturally occurring read-through transcription occurs between this locus and the neighboring locus NUDT3 (nudix (nucleoside diphosphate linked moiety X)-type motif 3).
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human RPS10 (NP_001190174.1).
NCBI Gene ID #:
6204
NCBI Official Name:
40S ribosomal protein S10
NCBI Official Symbol:
RPS10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 19kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P46783
User NOte:
Optimal dilutions for each application to be determined by the researcher.