ATP5A1 Antibody

ProSci
Product Code: PSI-19-918
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-918-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ATP5A1 Antibody (19-918) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded rat ovary using ATP5A1 antibody (19-918) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver cancer using ATP5A1 antibody (19-918) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using ATP5A1 antibody (19-918) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using ATP5A1 antibody (19-918) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of HeLa cells using ATP5A1 antibody (19-918) at dilution of 1:100 (60x lens). Blue: DAPI for nuclear staining.
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Confocal immunofluorescence analysis of Hela cells using ATP5A1 antibody (19-918) at dilution of 1:200. Blue: DAPI for nuclear staining.
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Confocal immunofluorescence analysis of U-2OS cells using ATP5A1 antibody (19-918) at dilution of 1:200. Blue: DAPI for nuclear staining.
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Immunoprecipitation analysis of 200ug extracts of MCF-7 cells using 1ug ATP5A1 antibody (19-918). Western blot was performed from the immunoprecipitate using ATP5A1 antibody (19-918) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using ATP5A1 Antibody (19-918) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat ovary using ATP5A1 antibody (19-918) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using ATP5A1 antibody (19-918) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using ATP5A1 antibody (19-918) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using ATP5A1 antibody (19-918) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using ATP5A1 antibody (19-918) at dilution of 1:100 (60x lens). Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of Hela cells using ATP5A1 antibody (19-918) at dilution of 1:200. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of U-2OS cells using ATP5A1 antibody (19-918) at dilution of 1:200. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of MCF-7 cells using 1ug ATP5A1 antibody (19-918). Western blot was performed from the immunoprecipitate using ATP5A1 antibody (19-918) at a dilition of 1:1000.

Further Information

Additional Names:
ATP5A1, ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle, ATP5A, ATP5AL2, ATPM, MOM2, OMR, ORM, hATP1, ATP synthase alpha chain, mitochondrial, ATP synthase subunit alpha, mitochondrial, ATP synthase, H+ transportin
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100

IP: 1:50 - 1:200
Background:
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ATP5A1 (NP_004037.1).
NCBI Gene ID #:
498
NCBI Official Name:
ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
NCBI Official Symbol:
ATP5A1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 53kDa
Purification:
Affinity purification
Research Area:
Neuroscience, Signal Transduction
Swissprot #:
P25705
User NOte:
Optimal dilutions for each application to be determined by the researcher.