RPS3A Antibody

ProSci
Product Code: PSI-19-919
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-919-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using RPS3A antibody (19-919) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat brain using RPS3A Antibody (19-919) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver cancer using RPS3A Antibody (19-919) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using RPS3A Antibody (19-919) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of MCF-7 cells using RPS3A antibody (19-919). Blue: DAPI for nuclear staining.
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Immunoprecipitation analysis of 200ug extracts of MCF7 cells using 3ug RPS3A antibody (19-919). Western blot was performed from the immunoprecipitate using RPS3A antibody (19-919) at a dilition of 1:500.

Western blot analysis of extracts of various cell lines, using RPS3A antibody (19-919) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using RPS3A Antibody (19-919) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using RPS3A Antibody (19-919) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using RPS3A Antibody (19-919) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using RPS3A antibody (19-919). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of MCF7 cells using 3ug RPS3A antibody (19-919). Western blot was performed from the immunoprecipitate using RPS3A antibody (19-919) at a dilition of 1:500.

Further Information

Additional Names:
40S ribosomal protein S3a {ECO:0000255|HAMAP-Rule:MF_03122}, v-fos transformation effector protein, Fte-1, RPS3A {ECO:0000255|HAMAP-Rule:MF_03122}, FTE1, MFTL
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

IP: 1:50 - 1:100
Background:
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S3AE family of ribosomal proteins. It is located in the cytoplasm. Disruption of the gene encoding rat ribosomal protein S3a, also named v-fos transformation effector protein, in v-fos-transformed rat cells results in reversion of the transformed phenotype. This gene is co-transcribed with the U73A and U73B small nucleolar RNA genes, which are located in its fourth and third introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human RPS3A (NP_000997.1).
NCBI Gene ID #:
6189
NCBI Official Name:
40S ribosomal protein S3a {ECO:0000255|HAMAP-Rule:MF_03122}
NCBI Official Symbol:
RPS3A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 35kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P61247
User NOte:
Optimal dilutions for each application to be determined by the researcher.