RPS12 Antibody

ProSci
Product Code: PSI-19-924
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-924-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using RPS12 antibody (19-924) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
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Immunohistochemistry of paraffin-embedded rat brain using RPS12 antibody (19-924) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast using RPS12 antibody (19-924) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using RPS12 antibody (19-924) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of MCF-7 cells using RPS12 antibody (19-924). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using RPS12 antibody (19-924) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat brain using RPS12 antibody (19-924) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using RPS12 antibody (19-924) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using RPS12 antibody (19-924) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using RPS12 antibody (19-924). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
40S ribosomal protein S12, RPS12
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:10 - 1:100
Background:
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S12E family of ribosomal proteins. It is located in the cytoplasm. Increased expression of this gene in colorectal cancers compared to matched normal colonic mucosa has been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-132 of human RPS12 (NP_001007.2).
NCBI Gene ID #:
6206
NCBI Official Name:
40S ribosomal protein S12
NCBI Official Symbol:
RPS12
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 15kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P25398
User NOte:
Optimal dilutions for each application to be determined by the researcher.