NCL Antibody

ProSci
Product Code: PSI-19-935
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-935-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using NCL antibody (19-935) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human gastric using NCL antibody (19-935) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using NCL antibody (19-935) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using NCL antibody (19-935) at dilution of 1:200 (40x lens).

Western blot analysis of extracts of various cell lines, using NCL antibody (19-935) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human gastric using NCL antibody (19-935) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using NCL antibody (19-935) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using NCL antibody (19-935) at dilution of 1:200 (40x lens).

Further Information

Additional Names:
NCL, C23
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 461-710 of human NCL (NP_005372.2).
NCBI Gene ID #:
4691
NCBI Official Name:
nucleolin
NCBI Official Symbol:
NCL
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 110kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P19338
User NOte:
Optimal dilutions for each application to be determined by the researcher.