TRIM69 Antibody

ProSci
Product Code: PSI-19-938
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-938-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 3
Western blot analysis of extracts of mouse liver, using TRIM69 antibody (19-938) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
2 / 3
Immunohistochemistry of paraffin-embedded human kidney using TRIM69 antibody (19-938) at dilution of 1:100 (40x lens).
3 / 3
Immunofluorescence analysis of A549 cells using TRIM69 antibody (19-938). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of mouse liver, using TRIM69 antibody (19-938) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human kidney using TRIM69 antibody (19-938) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using TRIM69 antibody (19-938). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
RNF36, Trif, HSD34, RNF36
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a member of the RING-B-box-coiled-coil (RBCC) family and encodes a protein with an N-terminal RING finger motif, a PRY domain and a C-terminal SPRY domain. The mouse ortholog of this gene is specifically expressed in germ cells at the round spermatid stages during spermatogenesis and, when overexpressed, induces apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-341 of human TRIM69 (NP_542783.2).
NCBI Gene ID #:
140691
NCBI Official Name:
tripartite motif containing 69
NCBI Official Symbol:
TRIM69
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 57kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Stem Cell
Swissprot #:
Q86WT6
User NOte:
Optimal dilutions for each application to be determined by the researcher.