EIF1AX Antibody

ProSci
Product Code: PSI-19-941
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-941-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using EIF1AX antibody (19-941) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 5
Immunohistochemistry of paraffin-embedded human stomach using EIF1AX antibody (19-941) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using EIF1AX antibody (19-941) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of C6 cells using EIF1AX Polyclonal Antibody (19-941) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH-3T3 cells using EIF1AX Polyclonal Antibody (19-941) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using EIF1AX antibody (19-941) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human stomach using EIF1AX antibody (19-941) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using EIF1AX antibody (19-941) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using EIF1AX Polyclonal Antibody (19-941) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using EIF1AX Polyclonal Antibody (19-941) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
EIF1A, EIF1AP1, EIF4C, eIF-1A, eIF-4C, eukaryotic translation initiation factor 1A, X-chromosomal, Putative eukaryotic translation initiation factor 1A, eIF-1A X isoform, eukaryotic translation initiation factor 1A, X chromosome, eukaryotic translation initiation factor 4C
Application Note:
WB: 1:200 - 1:2000

IHC: 1:100 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes an essential eukaryotic translation initiation factor. The protein is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 10-144 of human EIF1AX (NP_001403.1).
NCBI Gene ID #:
1964
NCBI Official Name:
eukaryotic translation initiation factor 1A X-linked
NCBI Official Symbol:
EIF1AX
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 16kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P47813
User NOte:
Optimal dilutions for each application to be determined by the researcher.