RBM3 Antibody

ProSci
Product Code: PSI-19-949
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-949-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 4
Western blot analysis of extracts of various cell lines, using RBM3 antibody (19-949) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
2 / 4
Immunohistochemistry of paraffin-embedded rat brain using RBM3 antibody (19-949) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded human endometrium cancer using RBM3 antibody (19-949) at dilution of 1:100 (40x lens).
4 / 4
Immunohistochemistry of paraffin-embedded mouse brain using RBM3 antibody (19-949) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using RBM3 antibody (19-949) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat brain using RBM3 antibody (19-949) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human endometrium cancer using RBM3 antibody (19-949) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using RBM3 antibody (19-949) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
RNA binding motif protein 3, OTTHUMP00000025802, OTTHUMP00000025800, RNPL, IS1-RNPL, RNA binding motif (RNP1, RRM) protein 3, RBM3
Application Note:
WB: 1:200 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene is a member of the glycine-rich RNA-binding protein family and encodes a protein with one RNA recognition motif (RRM) domain. Expression of this gene is induced by cold shock and low oxygen tension. A pseudogene exists on chromosome 1. Multiple alternatively spliced transcript variants that are predicted to encode different isoforms have been characterized although some of these variants fit nonsense-mediated decay (NMD) criteria.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-157 of human RBM3 (NP_006734.1).
NCBI Gene ID #:
5935
NCBI Official Name:
RNA binding motif (RNP1, RRM) protein 3
NCBI Official Symbol:
RBM3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 17kDa
Purification:
Affinity purification
Research Area:
Cancer
Swissprot #:
P98179
User NOte:
Optimal dilutions for each application to be determined by the researcher.