CELF1 Antibody

ProSci
Product Code: PSI-19-959
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-959-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CELF1 antibody (19-959) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.

Western blot analysis of extracts of various cell lines, using CELF1 antibody (19-959) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.

Further Information

Additional Names:
BRUNOL2, CUG-BP, CUGBP, CUGBP1, EDEN-BP, NAB5NAPOR, hNab5CUGBP Elav-like family member 1, 50 kDa nuclear polyadenylated RNA-binding protein, CELF-1, CUG RNA-binding protein, CUG triplet repeat RNA-binding protein 1, CUG-BP- and ETR-3-like factor 1, CUG-BP1, EDEN-BP homolog, RNA-binding protein BRUNOL-2, bruno-like 2, bruno-like protein 2, deadenylation factor CUG-BP, embryo deadenylation element binding protein, embryo deadenylation element-binding protein homolog, nuclear polyadenylated RNA-binding protein, 50-kD
Application Note:
WB: 1:200 - 1:2000
Background:
Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. This gene may play a role in myotonic dystrophy type 1 (DM1) via interactions with the dystrophia myotonica-protein kinase (DMPK) gene. Alternative splicing results in multiple transcript variants encoding different isoforms.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 108-483 of human CELF1 (NP_941989.1).
NCBI Gene ID #:
10658
NCBI Official Name:
CUGBP Elav-like family member 1
NCBI Official Symbol:
CELF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 52kDa
Purification:
Affinity purification
Research Area:
Neuroscience
Swissprot #:
Q92879
User NOte:
Optimal dilutions for each application to be determined by the researcher.