HNRNPA0 Antibody

ProSci
Product Code: PSI-19-983
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-983-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 4
Western blot analysis of extracts of various cell lines, using HNRNPA0 antibody (19-983) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
2 / 4
Immunohistochemistry of paraffin-embedded human esophagus using HNRNPA0 antibody (19-983) at dilution of 1:100 (40x lens).
3 / 4
Immunofluorescence analysis of L-929 cells using HNRNPA0 Polyclonal Antibody (19-983) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
4 / 4
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug HNRNPA0 antibody (19-983). Western blot was performed from the immunoprecipitate using HNRNPA0 antibody (19-983) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using HNRNPA0 antibody (19-983) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human esophagus using HNRNPA0 antibody (19-983) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of L-929 cells using HNRNPA0 Polyclonal Antibody (19-983) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug HNRNPA0 antibody (19-983). Western blot was performed from the immunoprecipitate using HNRNPA0 antibody (19-983) at a dilition of 1:1000.

Further Information

Additional Names:
HNRPA0, HNRPA0
Application Note:
WB: 1:500 - 1:1000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100

IP: 1:50 - 1:100
Background:
This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind RNAs, followed by a glycine-rich C-terminus.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human HNRNPA0 (NP_006796.1).
NCBI Gene ID #:
10949
NCBI Official Name:
heterogeneous nuclear ribonucleoprotein A0
NCBI Official Symbol:
HNRNPA0
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 37kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q13151
User NOte:
Optimal dilutions for each application to be determined by the researcher.