IGF2BP3 Antibody

ProSci
Product Code: PSI-22-006
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-006-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using IGF2BP3 antibody (22-006) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
2 / 3
Immunofluorescence analysis of U2OS cells using IGF2BP3 antibody (22-006) at dilution of 1:100. Blue: DAPI for nuclear staining.
3 / 3
Immunoprecipitation analysis of 100ug extracts of HepG2 cells using 3ug IGF2BP3 antibody (22-006). Western blot was performed from the immunoprecipitate using IGF2BP3 antibody (22-006) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using IGF2BP3 antibody (22-006) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunofluorescence analysis of U2OS cells using IGF2BP3 antibody (22-006) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 100ug extracts of HepG2 cells using 3ug IGF2BP3 antibody (22-006). Western blot was performed from the immunoprecipitate using IGF2BP3 antibody (22-006) at a dilition of 1:1000.

Further Information

Additional Names:
CT98, IMP-3, IMP3, KOC, KOC1, VICKZ3, insulin-like growth factor 2 mRNA-binding protein 3, IGF-II mRNA-binding protein 3, IGF2 mRNA-binding protein 3, KH domain containing protein overexpressed in cancer, VICKZ family member 3, cancer/testis antigen 98
Application Note:
WB: 1:500 - 1:1000

IF: 1:50 - 1:100

IP: 1:20 - 1:50
Background:
The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 300-579 of human IGF2BP3 (NP_006538.2).
NCBI Gene ID #:
10643
NCBI Official Name:
insulin like growth factor 2 mRNA binding protein 3
NCBI Official Symbol:
IGF2BP3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 70kDa
Purification:
Affinity purification
Research Area:
Cancer
Swissprot #:
O00425
User NOte:
Optimal dilutions for each application to be determined by the researcher.